Wikipedia
Search Wikipedia:

Latest topics
» Catalysis and its importance in chemical industry
Thu Nov 09, 2017 5:06 am by bejoy

» Cracking: Breaking up larger molecules
Thu Nov 09, 2017 5:05 am by bejoy

» What is Alkylation?
Fri Nov 03, 2017 12:35 am by bejoy

» An Ultimate Guide on Diamond
Thu Nov 02, 2017 2:15 am by bejoy

» Chemistry Running Behind Anger
Mon Oct 30, 2017 12:31 am by bejoy

» The Discovery of Four New Elements in the Periodic Table
Wed Oct 25, 2017 1:23 am by bejoy

» Carbonic Acid: Occurrence, Preparation, Properties and Uses
Mon Oct 23, 2017 1:39 am by bejoy

» Manufacturing of alumina through Bayer process
Tue Oct 17, 2017 1:16 am by bejoy

» Lithium batteries to run at ultra-low temperatures
Thu Oct 12, 2017 12:54 am by bejoy

November 2017
MonTueWedThuFriSatSun
  12345
6789101112
13141516171819
20212223242526
27282930   

Calendar Calendar

Free Hit Counter

Determination of Total D-Gluconic Acid in Honey

View previous topic View next topic Go down

Determination of Total D-Gluconic Acid in Honey

Post  sherryfu on Wed Apr 14, 2010 1:13 am

To synthesize functionalized poly(lactic acid-co-glycolic acid)-based polyesters for biomedical and pharmaceutical applications such as controlled drug delivery, d-gluconic acid was considered as an interesting source of comonomer. Accordingly D-Gluconic-acid was used to synthesize novel 1,4-dioxane-2,5-diones with protected hydroxyl groups, namely 3-dioxane-2,5-dione and 3-methyl-6-dioxane-2,5-dione.

The solution of honey was adjusted to pH 10.5 with 0.1 N KOH; 10 min at room temperature was allowed for hydrolysis of D-glucono-δ-lactone to D-gluconic acid. After this, it was necessary to adjust to test pH (7.Cool with 0.1 N HCl. With these conditions, there were no observed interference effects.

The enzymatic determination was measured spectrophotometrically at 340 nm, using gluconate kinase and 6-phosphogluconate dehydrogenase. The method combines precision (% CV was 0.30, at worst), good recovery (99.8%), simplicity, and low cost because this cost was reduced by 60% using a microtest. The total D-Gluconic-acidcontent of the honeys analyzed ranges between 3.91 and 11.71 g/kg (mean = 7.37 g/kg), which is in keeping with value ranges obtained by other authors using HPLC methods.

sherryfu

Posts : 50
Join date : 2010-04-12

View user profile

Back to top Go down

View previous topic View next topic Back to top

- Similar topics

 
Permissions in this forum:
You cannot reply to topics in this forum