Wikipedia
Search Wikipedia:

Latest topics
» Catalysis and its importance in chemical industry
Thu Nov 09, 2017 5:06 am by bejoy

» Cracking: Breaking up larger molecules
Thu Nov 09, 2017 5:05 am by bejoy

» What is Alkylation?
Fri Nov 03, 2017 12:35 am by bejoy

» An Ultimate Guide on Diamond
Thu Nov 02, 2017 2:15 am by bejoy

» Chemistry Running Behind Anger
Mon Oct 30, 2017 12:31 am by bejoy

» The Discovery of Four New Elements in the Periodic Table
Wed Oct 25, 2017 1:23 am by bejoy

» Carbonic Acid: Occurrence, Preparation, Properties and Uses
Mon Oct 23, 2017 1:39 am by bejoy

» Manufacturing of alumina through Bayer process
Tue Oct 17, 2017 1:16 am by bejoy

» Lithium batteries to run at ultra-low temperatures
Thu Oct 12, 2017 12:54 am by bejoy

November 2017
MonTueWedThuFriSatSun
  12345
6789101112
13141516171819
20212223242526
27282930   

Calendar Calendar

Free Hit Counter

Effect of 2-Chloroadenosine on Renal Oxidative Stress in Diabetic Wistar and Shr

View previous topic View next topic Go down

Effect of 2-Chloroadenosine on Renal Oxidative Stress in Diabetic Wistar and Shr

Post  sherryfu on Wed Aug 18, 2010 3:42 am

To investigate the effect of 2-chloroadenosine (2CADO) and 1,3-dipropyl-8-sulfophenylxanthine (DPSPX) on renal oxidative stress of diabetic Wistar and SHR rats.Design and Methods: On day 0, male Wistar (240–300 g) and SHR (12 weeks) rats were i.p. injected with streptozotocin (65 mg/Kg; STZ) or vehicle (SHAM). On day 14, osmotic mini-pumps were i.p. implanted for 2CADO (5 mg/Kg/d; CADO) or DPSPX (90 μg/Kg/h; DPSPX) administration for 7 days. Catheters were implanted for systolic blood pressure (SBP) measurement. Plasma, kidney and 24h-urine samples were collected at the end of the study. Glucose and hydrogen peroxide (H2O2) were quantified with commercial kits. Catalase (Cat) activity was measured by a spectrophotometric assay and thiobarbituric acid reactive substances (TBARS) by a colorimetric assay. Statistical comparisons were made by Student t-test and ANOVA followed by Dunnett's Multiple Comparison test as appropriate.Results: Results are summarized in the Table below. Diabetes increased plasma glucose in both strains. Treatment with 2CADO or DPSPX decreased plasma glucose in STZ-Wistar rats. Diabetes increased both Cat activity and H2O2 production in the medula of Wistar rats and treatment with 2CADO attenuated this increase. In SHR rats, medulary production of H2O2 was decreased after induction of diabetes and even further after 2-CADO administration. Urinary excretion of H2O2 did not change in both strains, with either treatment. Urinary TBARS increased with the induction of diabetes and this increase was attenuated in both strains by 2CADO. SBP decreased in SHR rats after diabetes induction and treatment with 2-CADO caused a further decrease.Conclusions: STZ-induced diabetes is associated with increased renal oxidative stress in Wistar and SHR rats. Treatment with 2CADO reduced oxidative stresse in Wistar but not SHR rats, although this protective effect seems to be mediated by different mechanisms. Further studies will help to clarify the differences observed between the two strains.

sherryfu

Posts : 50
Join date : 2010-04-12

View user profile

Back to top Go down

Effect of 2-Chloroadenosine

Post  jyoti on Mon Oct 04, 2010 12:41 pm

After analysing a lot the final result that is output is that : Diabetes increased plasma glucose in both strains.
Treatment with some medices such as 2CADO or DPSPX decreased plasma glucose in STZ-Wistar rats. So, If the diabetes increased then both Cat activity and H2O2 production in the rats as well as the treatment with 2CADO attenuated this increase. Urinary excretion did not change in both strains, with either treatment. These types of concepts are new for these types of concepts you need to research a lot and go through tutarials carbonyl chemistry, carbonyl chemistry tutorial which will help you to increase some of your knowledge.

jyoti

Posts : 63
Join date : 2010-08-25

View user profile

Back to top Go down

View previous topic View next topic Back to top

- Similar topics

 
Permissions in this forum:
You cannot reply to topics in this forum